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KMID : 0861020220370050009
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Korea Journal of Herbology
2022 Volume.37 No. 5 p.9 ~ p.16
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A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis
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Kim Wook-Jin
Choi Go-Ya
Noh Su-Min
Moon Byeong-Cheol
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Abstract
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Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber
is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry
of Food and Drug Safety(MFDS).
Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and
genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits
of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium
flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation,
respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification
using species-specific primers. DNA barcoding analysis used sequence information of the matK region.
Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species ‘P.
ternata’ of genuine ‘Pinelliae Tuber’, and 23 were identified as adulterant species ‘P. pedatisecta’. Unlike this, the
genetic identification was identified as the original species ‘P. ternata’ in all 50 samples in the SCAR marker and
matK regional sequence analysis.
Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is
imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of
the morphological mutant. 1
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KEYWORD
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Pinellia ternata, Pinelliae Tuber, Morphology, SCAR marker, DNA barcoding analysis
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